Journal: PLoS ONE

Article Title: RNA Silencing of Mcl-1 Enhances ABT-737-Mediated Apoptosis in Melanoma: Role for a Caspase-8-Dependent Pathway

doi: 10.1371/journal.pone.0006651

Figure Lengend Snippet: Lox IMVI cells were pretreated with the recombinant chimera Fas/Fc, TNF-R1/Fc, TRAIL-R1/Fc or a BSA control (1 µg/mL) at the indicative doses prior to treatment with 10 nM DsiRNA, 10 µM ABT-737 for 24 hours. For the triple combination of inhibitors, the indicated dose represents the dose of each individual inhibitor. Viability was assessed by MTT assay. Data represent mean±SEM (n = 4).

Article Snippet: Caspase-10 inhibitor Z-AEVD-FMK and recombinant chimera (Fas/Fc, TNF-R1/Fc, TRAIL-R1/Fc) were from R&D Systems (Minneapolis, MN).

Techniques: Recombinant, Control, MTT Assay

Journal: Gene therapy

Article Title: Intercellular trafficking and enhanced in vivo antitumour activity of a non-virally delivered P27-VP22 fusion protein.

doi: 10.1038/sj.gt.3301904

Figure Lengend Snippet: Figure 6 No infiltration of NK or monocytes cells or massive induction of apoptosis were observed after treatment of the tumours by P27VP22. Tumours were excised from nude mice 24 h after the last intratumoural injection of 10 mg DNA vector encoding Luc, P27, P27VP22 or VP22 as in Figure 5. Five micrometer tumour slices were then immunostained with anti-CD11b antibody (a) or an anti-active form of caspase 3 (b) and nuclei were counterstained with haematoxylin staining. P27 stands for p27Kip1.

Article Snippet: After saturation with 5% BSA for 30 min, rabbit anti-human Caspase three Active (R&D Systems) at 1/ 5000 in BSA 1% was added overnight at 41C.

Techniques: Injection, Plasmid Preparation, Staining

Journal: Cell Reports Medicine

Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer

doi: 10.1016/j.xcrm.2026.102630

Figure Lengend Snippet: dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, DR4, DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .

Article Snippet: anti-caspase-10 antibody , Proteintech , 14311-1-AP, RRID: AB_2290777.

Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Methylated DNA Immunoprecipitation, ChIP-qPCR